The Ionic system uses isotachophoresis (ITP) to isolate, purify, and concentrate genomic DNA and RNA from cells, tissue, and FFPE samples with Ionic G2 chemistry. DNA and RNA samples prepared by the system require just a few minutes of hands-on time per sample.
The Ionic system outperforms most nucleic acid extraction and purification methods:
By applying an electric field across the length of the Ionic Chip microchannels, the Ionic system separates and concentrates nucleic acid between buffers with higher and lower mobilities. Impurities fall behind the low mobility buffer and are separated from target nucleic acids. As target nucleic acids pass through the channel, an integrated sensor stops the current once nucleic acids reach the extraction well.
The workflow requires no binding, stripping, or washing from fixed surfaces for higher yields, higher quality nucleic acids, and ultimately, better data for your research.
Isotachophoresis (ITP) separates and concentrates charged molecules in solution solely based on their electrophoretic mobility. Biological samples are gently lysed and added to the Ionic Fluidic Chip. An electric field is then applied to the chip and the nucleic acid is isolated in its natural, native form. The nucleic acid is not denatured or dehydrated, and there’s no binding to, or stripping from, fixed surfaces. The result is a higher yield of pure nucleic acid that is less fragmented and free from bead or wash buffer contamination.
Extract Up to 4.5x More Nucleic Acid with Ionic G2
A vast majority of clinical samples used in oncology research are stored as FFPE tissues, which often contain degraded or fragmented nucleic acid. Conventional extraction methods are labor intensive and can further damage nucleic acid during the extraction and purification process.
The Ionic system simplifies and accelerates nucleic acid purification, resulting in higher yields of higher quality DNA.
A simplified workflow minimizes opportunities for material loss and errors. The FFPE protocols greatly simplify the processing 3 minutes per sample and enables working directly from scrolls. The protocol also eliminates the need for of FFPE samples. For example, the Ionic FFPE to Pure a separate paraffin removal step.